RESUMO
Objective: To investigate the relationship of vitamin D deficiency and risk of AMI in a Pakistani population, and to find out any association between vitamin D binding protein [VDBP] genotypes and risk of AMI in this population
Methods: In a comparative cross-sectional study, 246 patients [age: 20-70 years; 171 males and 75 females] with first AMI were enrolled with informed consent. Similarly, 345 healthy adults [230 males and 115 females] were enrolled as controls. Their fasting serum samples were analyzed for 25 [OH] vitamin D, lipids and other biomarkers using kit methods, while DNA was analyzed for VDBP genotypes using PCR-RFLP based methods. Chi-squared test and logistic regression were used for association of vitamin D deficiency and VDBP genotypes with AMI
Results: Mean serum concentration of 25[OH] vitamin D was significantly lower in AMI patients compared to healthy subjects [p=0.015] and percent vitamin D deficiency was higher in AMI patients compared to healthy subjects [p=0.003]. VDBP IF-IF genotype was positively associated with the risk of AMI in subject above 45 years after adjusting for potential confounders [OR = 9.86; 95% CI=1.16 to 83.43]
Conclusion: Vitamin D deficiency and VDBP IF-IF genotype are associated with AMI in Pakistani adults
RESUMO
Objective: to assess the pattern of plasma lipoproteins in CAD patients including comparison between diabetic and non-diabetic subgroups
Methods: this case control study was conducted at National Institute of Cardiovascular Diseases [NICVD] Karachi on patients admitted for CABG from June, 2000 to June, 2001. A total of 100 patients and 100 healthy subjects were enrolled during the study. 38 patients [38%] were diabetic with mean age of 46.16 years and 62 patients [62%] were non-diabetic with mean age of 45.26 years. Serum lipids, apolipoproteins A, apolipoproteins B and Lp [a] of each subject were analyzed. One way ANOVA and 95% confidence interval for mean were applied to find the association of CAD with age, BMI, glucose, cholesterol, triacylglycerol's, HDL-c, LDL-c, and Lp [a]
Result: CABG patients had significantly higher blood TAG levels than controls. Similar results were observed for HDL-c, which was significantly lower when compared with control subjects. Apo-B and Lp [a] in controls were significantly lower than both diabetic and non-diabetic CAD patients. The level of Apo-B in diabetic CAD was significantly higher when compared with control subjects